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CaMKII alpha Monoclonal antibody

CaMKII alpha Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E3B3

Cat No : 66843-1-Ig

Print datasheet

Synonyms

CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CAMKA, KIAA0968

验证数据展示

  • IF Staining of SH-SY5Y using 66843-1-Ig

    IF Staining of SH-SY5Y using 66843-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CAMK2 antibody (66843-1-Ig, Clone: 1E3B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis using 66843-1-Ig

    WB analysis using 66843-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of mouse brain using 66843-1-Ig

    IHC staining of mouse brain using 66843-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66843-1-Ig

    IHC staining of mouse brain using 66843-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 66843-1-Ig

    WB analysis using 66843-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inpig brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue, rabbit brain tissue, rat rain tissue
Positive IHC detected inmouse brain tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66843-1-Ig targets CaMKII alpha in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityrat
Immunogen CaMKII alpha fusion protein Ag4736 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calcium/calmodulin-dependent protein kinase II alpha
Synonyms CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CAMKA, KIAA0968
Calculated Molecular Weight 478 aa, 54 kDa
Observed Molecular Weight 45-60 kDa
GenBank Accession NumberBC040457
Gene Symbol CAMK2A
Gene ID (NCBI) 815
RRIDAB_2882184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UQM7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody can recognize all the members of the CAMK2 family.

实验方案

Product Specific Protocols
WB protocol for CaMKII alpha antibody 66843-1-IgDownload protocol
IHC protocol for CaMKII alpha antibody 66843-1-IgDownload protocol
IF protocol for CaMKII alpha antibody 66843-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Aging (Albany NY)

Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway.

Authors - Ting-Ting Chen
View Article