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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • IF Staining of SH-SY5Y using 66843-1-Ig (same clone as 66843-1-PBS)

    IF Staining of SH-SY5Y using 66843-1-Ig (same clone as 66843-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CAMK2 antibody (66843-1-Ig, Clone: 1E3B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)

    WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)

    IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)

    IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)

    WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

CAMK2 Monoclonal antibody, PBS Only

CAMK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66843-1-PBS

产品说明书

CloneNo. 1E3B3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMKA, CaMKII alpha, CaMK-II subunit alpha

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66843-1-PBS targets CAMK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 CAMK2 fusion protein Ag4736 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase II alpha
别名 CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMKA, CaMKII alpha, CaMK-II subunit alpha
计算分子量 478 aa, 54 kDa
观测分子量 45-60 kDa
GenBank蛋白编号BC040457
基因名称 CaMKII alpha
Gene ID (NCBI) 815
RRIDAB_2882184
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UQM7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody can recognize all the members of CAMK2 family.