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验证数据展示

  • WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

    WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

  • WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

    WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 68224-1-Ig (same clone as 68224-1-PBS)

    IF Staining of A549 using 68224-1-Ig (same clone as 68224-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CA12 antibody (68224-1-Ig, Clone: 3E1D8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

CA12 Monoclonal antibody, PBS Only

CA12 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68224-1-PBS

产品说明书

CloneNo. 3E1D8

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, Indirect ELISA

别名

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CA12 Ready-To-Use IHC Kit. CA12 primary antibody included.

产品信息

68224-1-PBS targets CA12 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag7235

Product name: Recombinant human CA12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 27-293 aa of BC000278

Sequence: VNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQGI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 carbonic anhydrase XII
别名 CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3
计算分子量 39 kDa
观测分子量38-45 kDa
GenBank蛋白编号BC000278
基因名称 CA12
Gene ID (NCBI) 771
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO43570
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

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