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验证数据展示

  • WB analysis using 68224-1-Ig

    WB analysis using 68224-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68224-1-Ig

    WB analysis using 68224-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 68224-1-Ig

    IF Staining of A549 using 68224-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CA12 antibody (68224-1-Ig, Clone: 3E1D8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CA12 Monoclonal antibody

CA12 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68224-1-Ig

产品说明书

CloneNo. 3E1D8

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HeLa cells
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy CA12 Ready-To-Use IHC Kit. CA12 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68224-1-Ig targets CA12 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag7235

Product name: Recombinant human CA12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 27-293 aa of BC000278

Sequence: VNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQGI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 carbonic anhydrase XII
别名 CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3
计算分子量 39 kDa
观测分子量38-45 kDa
GenBank蛋白编号BC000278
基因名称 CA12
Gene ID (NCBI) 771
RRIDAB_2935312
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO43570
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

实验方案

Product Specific Protocols
IF protocol for CA12 antibody 68224-1-IgDownload protocol
WB protocol for CA12 antibody 68224-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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