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验证数据展示

  • WB analysis using 14496-1-AP (same clone as 14496-1-PBS)

    WB analysis using 14496-1-AP (same clone as 14496-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14496-1-AP (C9orf78 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 14496-1-AP (same clone as 14496-1-PBS)

    IP experiment of HepG2 using 14496-1-AP (same clone as 14496-1-PBS)

    IP result of anti-C9orf78 (IP:14496-1-AP, 4ug; Detection:14496-1-AP 1:500) with HepG2 cells lysate 2200 ug. This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 14496-1-AP (same clone as 14496-1-PBS)

    IHC staining of human stomach cancer using 14496-1-AP (same clone as 14496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14496-1-AP (C9orf78 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14496-1-AP (same clone as 14496-1-PBS)

    IF Staining of HepG2 using 14496-1-AP (same clone as 14496-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C9orf78 antibody (14496-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

C9orf78 Polyclonal antibody, PBS Only

C9orf78 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14496-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

TLS1, HSPC220, Hepatocellular carcinoma-associated antigen 59, HCA59, CSU2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14496-1-PBS targets C9orf78 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5919

Product name: Recombinant human C9orf78 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-289 aa of BC007664

Sequence: MPVVRKIFRRRRGDSESEEDEQDSEEVRLKLEETREVQNLRKRPNGVSAVALLVGEKVQEETTLVDDPFQMKTGGMVDMKKLKERGKDKISEEEDLHLGTSFSAETNRRDEDADMMKYIETELKKRKGIVEHEEQKVKPKNAEDCLYELPENIRVSSAKKTEEMLSNQMLSGIPEVDLGIDAKIKNIISTEDAKARLLAEQQNKKKDSETSFVPTNMAVNYVQHNRFYHEELNAPIRRNKEEPKARPLRVGDTEKPEPERSPPNRKRPANEKATDDYHYEKFKKMNRRY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 78
别名 TLS1, HSPC220, Hepatocellular carcinoma-associated antigen 59, HCA59, CSU2
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC007664
基因名称 C9orf78
Gene ID (NCBI) 51759
RRIDAB_10644338
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZ63
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The function of C9orf78 remains largely unknown. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein. This antibody works well in WB, IHC and IF applications.

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