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验证数据展示

  • WB analysis using 14496-1-AP

    WB analysis using 14496-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14496-1-AP (C9orf78 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 14496-1-AP

    IP experiment of HepG2 using 14496-1-AP

    IP result of anti-C9orf78 (IP:14496-1-AP, 4ug; Detection:14496-1-AP 1:500) with HepG2 cells lysate 2200 ug.

  • IHC staining of human stomach cancer using 14496-1-AP

    IHC staining of human stomach cancer using 14496-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14496-1-AP (C9orf78 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 14496-1-AP

    IF Staining of HepG2 using 14496-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C9orf78 antibody (14496-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

C9orf78 Polyclonal antibody

C9orf78 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14496-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

TLS1, HSPC220, Hepatocellular carcinoma-associated antigen 59, HCA59, CSU2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman stomach cancer tissue, human bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14496-1-AP targets C9orf78 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 C9orf78 fusion protein Ag5919 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 78
别名 TLS1, HSPC220, Hepatocellular carcinoma-associated antigen 59, HCA59, CSU2
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC007664
基因名称 C9orf78
Gene ID (NCBI) 51759
RRIDAB_10644338
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZ63
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The function of C9orf78 remains largely unknown. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein. This antibody works well in WB, IHC and IF applications.

实验方案

Product Specific Protocols
WB protocol for C9orf78 antibody 14496-1-APDownload protocol
IHC protocol for C9orf78 antibody 14496-1-APDownload protocol
IF protocol for C9orf78 antibody 14496-1-APDownload protocol
IP protocol for C9orf78 antibody 14496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols