验证数据展示
经过测试的应用
| Positive FC detected in | human peripheral blood leukocytes |
Planning an IHC experiment? We recommend our IHCeasy C5AR1 Ready-To-Use IHC Kit. C5AR1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-FcA98226 targets C5AR1/CD88 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | complement component 5a receptor 1 |
| 别名 | C5AR, C5AR1, C5A, C5a anaphylatoxin chemotactic receptor 1, C5a R |
| 计算分子量 | 39kDa |
| GenBank蛋白编号 | NM_001736.4 |
| 基因名称 | C5aR |
| Gene ID (NCBI) | 728 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PE C5AR1/CD88 antibody PE-FcA98226 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |