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CoraLite®594-conjugated C5 Monoclonal antibody

C5 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G8C9

Cat No : CL594-66634

Print datasheet

Synonyms

C5, Complement C5, complement component 5, CPAMD4, FLJ17816, FLJ17822

验证数据展示

  • IF Staining of HepG2 using CL594-66634

    IF Staining of HepG2 using CL594-66634

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 C5 antibody (CL594-66634, Clone: 2G8C9 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-66634

    FC experiment of HepG2 using CL594-66634

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human C5 (CL594-66634, Clone:2G8C9) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66634-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66634 targets C5 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human
Immunogen C5 fusion protein Ag17882 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name complement component 5
Synonyms C5, Complement C5, complement component 5, CPAMD4, FLJ17816, FLJ17822
Calculated Molecular Weight 1676 aa, 188 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC113740
Gene Symbol C5
Gene ID (NCBI) 727
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01031
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 C5 antibody CL594-66634Download protocol
FC protocol for CL594 C5 antibody CL594-66634Download protocol
Standard Protocols
Click here to view our Standard Protocols