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验证数据展示

  • IF Staining of HepG2 using CL488-66634

    IF Staining of HepG2 using CL488-66634

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 C5 antibody (CL488-66634, Clone: 2G8C9 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66634

    FC experiment of HepG2 using CL488-66634

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human C5 (CL488-66634, Clone:2G8C9) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated C5 Monoclonal antibody

C5 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66634

产品说明书

CloneNo. 2G8C9

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

2G8C9, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, Complement C5, complement component 5, CPAMD4

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66634-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66634 targets C5 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag17882

Product name: Recombinant human C5, C5a protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1418-1676 aa of BC113740

Sequence: GSSHAVMDISLPTGISANEEDLKALVEGVDQLFTDYQIKDGHVILQLNSIPSSDFLCVRFRIFELFEVGFLSPATFTVYEYHRPDKQCTMFYSTSNIKIQKVCEGAACKCVEADCGQMQEELDLTISAETRKQTACKPEIAYAYKVSITSITVENVFVKYKATLLDIYKTGEAVAEKDSEITFIKKVTCTNAELVKGRQYLIMGKEALQIKYNFSFRYIYPLDSLTWIEYWPRDTTCSSCQAFLANLDEFAEDIFLNGC

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component 5
别名 2G8C9, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, Complement C5, complement component 5, CPAMD4
计算分子量 1676 aa, 188 kDa
观测分子量 115 kDa
GenBank蛋白编号BC113740
基因名称 C5
Gene ID (NCBI) 727
RRIDAB_2934483
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01031
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections. This antibody specifically recognizes the C5 alpha chain and C5b.(PMID: 20010915; PMID: 8011297; PMID: 6554279)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 C5 antibody CL488-66634Download protocol
IF protocol for CL Plus 488 C5 antibody CL488-66634Download protocol
Standard Protocols
Click here to view our Standard Protocols
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