验证数据展示
经过测试的应用
| Positive WB detected in | human blood |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85782-2-RR targets C4b Alpha Chain/C4b-B/C4d-B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | complement component 4B (Chido blood group) |
| 别名 | C4B, C4, C4B1, C4B12, C4B2 |
| 计算分子量 | 193 kDa |
| 观测分子量 | 95 kDa |
| GenBank蛋白编号 | NM_000592 |
| 基因名称 | C4B |
| Gene ID (NCBI) | 721 |
| RRID | AB_3744369 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P0C0L5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single-chain precursor, which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular weights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1116-1128aa of human C4b protein can recognize C4b precursor, C4b alpha chain, C4b-B, and C4d-B.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for C4b Alpha Chain/C4b-B/C4d-B antibody 85782-2-RR | Download protocol |
| WB protocol for C4b Alpha Chain/C4b-B/C4d-B antibody 85782-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
