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验证数据展示

  • WB analysis of human blood using 85782-2-RR (same clone as 85782-2-PBS)

    WB analysis of human blood using 85782-2-RR (same clone as 85782-2-PBS)

    Human blood was subjected to SDS PAGE followed by western blot with 85782-2-RR (C4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85782-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85782-2-RR (same clone as 85782-2-PBS)

    IF Staining of HepG2 using 85782-2-RR (same clone as 85782-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C4B antibody (85782-2-RR, Clone: 250030C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85782-2-PBS in a different storage buffer formulation.

C4b Alpha Chain/C4b-B/C4d-B Recombinant monoclonal antibody, PBS Only

C4b Alpha Chain/C4b-B/C4d-B Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85782-2-PBS

产品说明书

CloneNo. 250030C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

C4B, C4, C4B1, C4B12, C4B2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85782-2-PBS targets C4b Alpha Chain/C4b-B/C4d-B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement component 4B (Chido blood group)
别名 C4B, C4, C4B1, C4B12, C4B2
计算分子量 193 kDa
观测分子量95 kDa
GenBank蛋白编号NM_000592
基因名称 C4B
Gene ID (NCBI) 721
RRIDAB_3744369
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP0C0L5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single-chain precursor, which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular weights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1116-1128aa of human C4b protein can recognize C4b precursor, C4b alpha chain, C4b-B, and C4d-B.

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