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验证数据展示

  • IF Staining of A431 using CL488-85147-3

    IF Staining of A431 using CL488-85147-3

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 C1orf77 antibody (CL488-85147-3, Clone: 242770H3 ) at dilution of 1:200, CL594-Phalloidin (red).

  • IF Staining of HeLa using CL488-85147-3

    IF Staining of HeLa using CL488-85147-3

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 C1orf77 antibody (CL488-85147-3, Clone: 242770H3 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated C1orf77 Recombinant antibody

C1orf77 Recombinant Antibody for IF/ICC
Cat No. CL488-85147-3

产品说明书

CloneNo. 242770H3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

CHTOP, FOP, 242770H3, Friend of PRMT1 protein, PP7704

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells, A431 cells
For other applications, we recommend the unconjugated version of this antibody, 85147-3-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-85147-3 targets C1orf77 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 C1orf77 fusion protein Ag26580 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 1 open reading frame 77
别名 CHTOP, FOP, 242770H3, Friend of PRMT1 protein, PP7704
计算分子量 248 aa, 26 kDa
观测分子量26-30 kDa
GenBank蛋白编号BC002733
基因名称 C1orf77
Gene ID (NCBI) 26097
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y3Y2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1orf77, officially designated CHTOP (Chromatin Target of PRMT1), is a small nuclear protein encoded on human chromosome 1q21.3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 C1orf77 antibody CL488-85147-3Download protocol
Standard Protocols
Click here to view our Standard Protocols