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验证数据展示

  • WB analysis using 85147-3-RR

    WB analysis using 85147-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85147-3-RR (C1orf77 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 85147-3-RR

    IHC staining of human colon cancer using 85147-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85147-3-RR (C1orf77 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 85147-3-RR

    IHC staining of human colon cancer using 85147-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85147-3-RR (C1orf77 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach using 85147-3-RR

    IHC staining of human stomach using 85147-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 85147-3-RR (C1orf77 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 85147-3-RR

    IF Staining of HeLa using 85147-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C1orf77 antibody (85147-3-RR, Clone: 242770H3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of SH-SY5Y using 85147-3-RR

    IF Staining of SH-SY5Y using 85147-3-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using C1orf77 antibody (85147-3-RR, Clone: 242770H3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A431 using 85147-3-RR

    IF Staining of A431 using 85147-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C1orf77 antibody (85147-3-RR, Clone: 242770H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85147-3-RR

    Affinity and Kinetic Characterization of 85147-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85147-3-RR against Human C1orf77 were performed. The affinity constant is 9.63 nM.

C1orf77 Recombinant monoclonal antibody

C1orf77 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85147-3-RR

产品说明书

CloneNo. 242770H3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CHTOP, FOP, 242770H3, Friend of PRMT1 protein, PP7704

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HEK-293T cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells, HeLa cells, A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85147-3-RR targets C1orf77 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26580

Product name: Recombinant human C1orf77 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-118 aa of BC002733

Sequence: MSLRGQNLLRGGRAVAPRMGLRRGGVRGRGGPGRGGLGRGAMGRGGIGGRGRGMIGRGRGGFGGRGRGRGRGRGALARPVLTKEQLDNQLDAYMSKTKGHLDAELDAYMAQTDPETND

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 1 open reading frame 77
别名 CHTOP, FOP, 242770H3, Friend of PRMT1 protein, PP7704
计算分子量 248 aa, 26 kDa
观测分子量26-30 kDa
GenBank蛋白编号BC002733
基因名称 C1orf77
Gene ID (NCBI) 26097
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y3Y2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C1orf77 antibody 85147-3-RRDownload protocol
IHC protocol for C1orf77 antibody 85147-3-RRDownload protocol
IF protocol for C1orf77 antibody 85147-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols