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验证数据展示

  • WB analysis using 24820-1-AP

    WB analysis using 24820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24820-1-AP (C1RL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 24820-1-AP

    WB analysis using 24820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24820-1-AP (C1RL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 24820-1-AP

    IF Staining of U2OS using 24820-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using C1RL antibody (24820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

C1RL Polyclonal antibody

C1RL Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24820-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

C1r like protein, C1r LP, C1RL1, C1r-like protein, C1r-like serine protease analog protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma, HL-60 cells, K-562 cells, Ramos cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24820-1-AP targets C1RL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20324

Product name: Recombinant human C1RL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 41-195 aa of BC156780

Sequence: SVLLAQELPQQLTSPGYPEPYGKGQESSTDIKAPEGFAVRLVFQDFDLEPSQDCAGDSVTISFVGSDPSQFCGQQGSPLGRPPGQREFVSSGRSLRLTFRTQPSSENKTAHLHKGFLALYQTVAVNYSQPISEASRGSEAINAPGDNPAKVQNHC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component 1, r subcomponent-like
别名 C1r like protein, C1r LP, C1RL1, C1r-like protein, C1r-like serine protease analog protein
计算分子量 487 aa, 53 kDa
观测分子量53 kDa, 70 kDa
GenBank蛋白编号BC156780
基因名称 C1RL
Gene ID (NCBI) 51279
RRIDAB_3085758
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZP8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1r-like serine protease analog protein (CLSPa), also named as CIRL contains a CUB domain and a serine protease domain, possessing characteristic catalytic triad. C1RL is upregulated in glioblastoma (GBM) and has been proved to be a prognostic marker in hepatocellular carcinoma and renal cell cancer (PMID: 32993564). C1RL has a calculated molecular weight of 53 kDa, and this antibody can detect a band at 70 kDa due to possible N-glycosylation (PMID: 15358180).

实验方案

Product Specific Protocols
IF protocol for C1RL antibody 24820-1-APDownload protocol
WB protocol for C1RL antibody 24820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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