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验证数据展示

  • WB analysis of T-47D using 27569-1-AP (same clone as 27569-1-PBS)

    WB analysis of T-47D using 27569-1-AP (same clone as 27569-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 27569-1-AP (C1GALT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27569-1-PBS in a different storage buffer formulation.

  • WB analysis using 27569-1-AP (same clone as 27569-1-PBS)

    WB analysis using 27569-1-AP (same clone as 27569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27569-1-AP (C1GALT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27569-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 27569-1-AP (same clone as 27569-1-PBS)

    IF Staining of HepG2 using 27569-1-AP (same clone as 27569-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C1GALT1 antibody (27569-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27569-1-PBS in a different storage buffer formulation.

C1GALT1 Polyclonal antibody, PBS Only

C1GALT1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 27569-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

B3Gal T8, Beta 1,3 galactosyltransferase, C1GALT, Core 1 beta3 Gal T1, Core 1 O glycan T synthase

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27569-1-PBS targets C1GALT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26144

Product name: Recombinant human C1GALT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 263-363 aa of NM_020156

Sequence: MKETFHPFVPEHHLIKGYLPRTFWYWNYNYYPPVEGPGCCSDLAVSFHYVDSTTMYELEYLVYHLRPYGYLYRYQPTLPERILKEISQANKNEDTKVKLGNP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1
别名 B3Gal T8, Beta 1,3 galactosyltransferase, C1GALT, Core 1 beta3 Gal T1, Core 1 O glycan T synthase
计算分子量 42 kDa
观测分子量 42-45 kDa
GenBank蛋白编号NM_020156
基因名称 C1GALT1
Gene ID (NCBI) 56913
RRIDAB_2880912
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NS00
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C1GALT1 (Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1), also known as B3Gal-T8, catalyzes the transfer of galactose (Gal) from UDP-Gal to N-acetylgalactosamine (GalNAc) O-linked esters of threonine and serine residues of target proteins, including IgA1, to form the T antigen (PMID: 28209808). C1GalT1 is overexpressed in many common cancers includingcolon, breast, gastric, lung, head and neck, pancreatic, esophageal, prostate, and hepatocellular cancer (PMID: 34944925). C1GALT1 protein contains 363 amino acids and is a typeII transmembrane protein. Rate C1GalT1 is an 84/86KDa disulfide-bonded homodimer, but its active form is believed to be a 42/43 KDa monomeric protein (PMID: 34149900).

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