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验证数据展示

  • WB analysis of T-47D using 27569-1-AP

    WB analysis of T-47D using 27569-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 27569-1-AP (C1GALT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 27569-1-AP

    WB analysis using 27569-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27569-1-AP (C1GALT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

C1GALT1 Polyclonal antibody

C1GALT1 Polyclonal Antibody for WB, ELISA
Cat No. 27569-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA and More (1)

别名

B3Gal T8, Beta 1,3 galactosyltransferase, C1GALT, Core 1 beta3 Gal T1, Core 1 O glycan T synthase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMKN-45 cells, HepG2 cells, T-47D cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27569-1-AP targets C1GALT1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 C1GALT1 fusion protein Ag26144 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1
别名 B3Gal T8, Beta 1,3 galactosyltransferase, C1GALT, Core 1 beta3 Gal T1, Core 1 O glycan T synthase
计算分子量 42 kDa
观测分子量 42-45 kDa
GenBank蛋白编号NM_020156
基因名称 C1GALT1
Gene ID (NCBI) 56913
RRIDAB_2880912
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NS00
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1GALT1 (Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1), also known as B3Gal-T8, catalyzes the transfer of galactose (Gal) from UDP-Gal to N-acetylgalactosamine (GalNAc) O-linked esters of threonine and serine residues of target proteins, including IgA1, to form the T antigen (PMID: 28209808). C1GalT1 is overexpressed in many common cancers includingcolon, breast, gastric, lung, head and neck, pancreatic, esophageal, prostate, and hepatocellular cancer (PMID: 34944925). C1GALT1 protein contains 363 amino acids and is a typeII transmembrane protein. Rate C1GalT1 is an 84/86KDa disulfide-bonded homodimer, but its active form is believed to be a 42/43 KDa monomeric protein (PMID: 34149900).

实验方案

Product Specific Protocols
WB protocol for C1GALT1 antibody 27569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Int Immunopharmacol

The upregulation of miR-98-5p affects the glycosylation of IgA1 through cytokines in IgA nephropathy.

Authors - Di Liu
View Article
humanIHC

J Biomed Res

Genetic variants in C1GALT1 are associated with gastric cancer risk and immune infiltrations

Authors - Mengfan Guo
View Article