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验证数据展示

  • WB analysis using 84641-1-RR (same clone as 84641-1-PBS)

    WB analysis using 84641-1-RR (same clone as 84641-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84641-1-RR (C12orf51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84641-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84641-1-RR (same clone as 84641-1-PBS)

    IHC staining of human colon cancer using 84641-1-RR (same clone as 84641-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84641-1-RR (C12orf51 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84641-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84641-1-RR (same clone as 84641-1-PBS)

    IHC staining of human colon cancer using 84641-1-RR (same clone as 84641-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84641-1-RR (C12orf51 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84641-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84641-1-RR

    Affinity and Kinetic Characterization of 84641-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84641-1-RR against Human C12orf51 were performed. The affinity constant is below 1 pM.

C12orf51 Recombinant monoclonal antibody, PBS Only

C12orf51 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84641-1-PBS

产品说明书

CloneNo. 241815A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

HECTD4, 241815A12, HECT domain-containing protein 4, HECT-type E3 ubiquitin transferase HECTD4, KIAA0614

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84641-1-PBS targets C12orf51 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14614

Product name: Recombinant human C12orf51 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3723-3996 aa of BC006270

Sequence: TSFRKTLVGEPLDPEQDLQEADILTYNYVKKFESINDETELEALCAEIASQHLATESPDSPNKPCCRFTYLTMTGEEVELCSRGRHILVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGSIIPLQLLTMLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALEMFTQEELCKFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 12 open reading frame 51
别名 HECTD4, 241815A12, HECT domain-containing protein 4, HECT-type E3 ubiquitin transferase HECTD4, KIAA0614
计算分子量 3996 aa, 439 kDa
观测分子量500 kDa
GenBank蛋白编号BC006270
基因名称 C12orf51
Gene ID (NCBI) 283450
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y4D8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The HECT domain E3 ubiquitin protein ligase 4 (HECTD4), is also known as C12orf51. It may be involved in protein modification or ubiquitination (PMID: 26891264). In addition, genetic polymorphisms of C12orf51 are related to alcohol consumption and type 2 diabetes (T2D) (PMID: 38523829).

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