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验证数据展示

  • WB analysis using 84641-1-RR

    WB analysis using 84641-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84641-1-RR (C12orf51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 84641-1-RR

    IHC staining of human colon cancer using 84641-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84641-1-RR (C12orf51 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 84641-1-RR

    IHC staining of human colon cancer using 84641-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84641-1-RR (C12orf51 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84641-1-RR

    Affinity and Kinetic Characterization of 84641-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84641-1-RR against Human C12orf51 were performed. The affinity constant is below 1 pM.

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C12orf51 Recombinant monoclonal antibody

C12orf51 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84641-1-RR

产品说明书

CloneNo. 241815A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

HECTD4, 241815A12, HECT domain-containing protein 4, HECT-type E3 ubiquitin transferase HECTD4, KIAA0614

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA375 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84641-1-RR targets C12orf51 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14614

Product name: Recombinant human C12orf51 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3723-3996 aa of BC006270

Sequence: TSFRKTLVGEPLDPEQDLQEADILTYNYVKKFESINDETELEALCAEIASQHLATESPDSPNKPCCRFTYLTMTGEEVELCSRGRHILVAWENKDIYAAAIRSLRLRELQNVECVTAVRAGLGSIIPLQLLTMLSPLEMELRTCGLPYINLEFLKAHTMYQVGLMETDQHIEFFWGALEMFTQEELCKFIKFACNQERIPFTCPCKDGGPDTAHVPPYPMKIAPPDGTAGSPDSRYIRVETCMFMIKLPQYSSLEIMLEKLRCAIHYREDPLSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 12 open reading frame 51
别名 HECTD4, 241815A12, HECT domain-containing protein 4, HECT-type E3 ubiquitin transferase HECTD4, KIAA0614
计算分子量 3996 aa, 439 kDa
观测分子量500 kDa
GenBank蛋白编号BC006270
基因名称 C12orf51
Gene ID (NCBI) 283450
RRIDAB_3672100
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y4D8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The HECT domain E3 ubiquitin protein ligase 4 (HECTD4), is also known as C12orf51. It may be involved in protein modification or ubiquitination (PMID: 26891264). In addition, genetic polymorphisms of C12orf51 are related to alcohol consumption and type 2 diabetes (T2D) (PMID: 38523829).

实验方案

Product Specific Protocols
WB protocol for C12orf51 antibody 84641-1-RRDownload protocol
IHC protocol for C12orf51 antibody 84641-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols