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验证数据展示

  • WB analysis using 84641-1-RR

    WB analysis using 84641-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84641-1-RR (C12orf51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84641-1-RR

    Affinity and Kinetic Characterization of 84641-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84641-1-RR against Human C12orf51 were performed. The affinity constant is below 1 pM.

C12orf51 Recombinant antibody

C12orf51 Recombinant Antibody for WB, ELISA
Cat No. 84641-1-RR

产品说明书

CloneNo. 241815A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

HECTD4, Probable E3 ubiquitin-protein ligase HECTD4, KIAA0614, HECT-type E3 ubiquitin transferase HECTD4, HECT domain-containing protein 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, HeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84641-1-RR targets C12orf51 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 C12orf51 fusion protein Ag14614 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 12 open reading frame 51
别名 HECTD4, Probable E3 ubiquitin-protein ligase HECTD4, KIAA0614, HECT-type E3 ubiquitin transferase HECTD4, HECT domain-containing protein 4
计算分子量 3996 aa, 439 kDa
观测分子量500 kDa
GenBank蛋白编号BC006270
基因名称 C12orf51
Gene ID (NCBI) 283450
RRIDAB_3672100
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The HECT domain E3 ubiquitin protein ligase 4 (HECTD4), is also known as C12orf51. It may be involved in protein modification or ubiquitination (PMID: 26891264). In addition, genetic polymorphisms of C12orf51 are related to alcohol consumption and type 2 diabetes (T2D) (PMID: 38523829).

实验方案

Product Specific Protocols
WB protocol for C12orf51 antibody 84641-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols