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验证数据展示

  • WB analysis of A431 using 84725-5-RR (same clone as 84725-5-PBS)

    WB analysis of A431 using 84725-5-RR (same clone as 84725-5-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 84725-5-RR (C11orf68 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84725-5-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 84725-5-RR (same clone as 84725-5-PBS)

    WB analysis of PC-3 using 84725-5-RR (same clone as 84725-5-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 84725-5-RR (C11orf68 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84725-5-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 84725-5-RR (same clone as 84725-5-PBS)

    IHC staining of human stomach cancer using 84725-5-RR (same clone as 84725-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84725-5-RR (C11orf68 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84725-5-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 84725-5-RR (same clone as 84725-5-PBS)

    IF Staining of A431 using 84725-5-RR (same clone as 84725-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C11orf68 antibody (84725-5-RR, Clone: 242221C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84725-5-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84725-5-RR (same clone as 84725-5-PBS)

    FC experiment of U2OS using 84725-5-RR (same clone as 84725-5-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug C11orf68 Recombinant antibody (84725-5-RR, Clone:242221C11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84725-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84725-5-RR

    Affinity and Kinetic Characterization of 84725-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84725-5-RR against Human C11orf68 were performed. The affinity constant is 11.8 nM.

C11orf68 Recombinant monoclonal antibody, PBS Only

C11orf68 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84725-5-PBS

产品说明书

CloneNo. 242221C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

242221C11, BLES03, P5326, Protein p5326, UPF0696 protein C11orf68

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84725-5-PBS targets C11orf68 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36023

Product name: Recombinant human C11orf68 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-251 aa of BC010512

Sequence: MEPGEELEEEGSPGGREDGFTAEHLAAEAMAADMDPWLVFDARTTPATELDAWLAKYPPSQVTRYGDPGSPNSEPVGWIAVYGQGYSPNSGDVQGLQAAWEALQTSGRPITPGTLRQLAITHHVLSGKWLMHLAPGFKLDHAWAGIARAVVEGRLQVAKVSPRAKEGGRQVICVYTDDFTDRLGVLEADSAIRAAGIKCLLTYKPDVYTYLGIYRANRWHLCPTLYESRFQLGGSARGSRVLDRANNVELT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 11 open reading frame 68
别名 242221C11, BLES03, P5326, Protein p5326, UPF0696 protein C11orf68
观测分子量35 kDa
GenBank蛋白编号BC010512
基因名称 C11orf68
Gene ID (NCBI) 83638
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H3H3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BLES03 (basophilic leukemia-expressed protein 03) is a human protein of unknown function. Recent studies have shown that BLES03 is a novel eukaryotic phosphothreonine cleavage enzyme involved in the biosynthesis of dehydrogenated amino acids.The crystal structure of BLES03 has been resolved by single-wavelength anomalous scattering (SAD) with a resolution of 2.5 Å.

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