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验证数据展示

  • FC experiment of HepG2 using CL647-20536

    FC experiment of HepG2 using CL647-20536

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Beta Actin (CL647-20536) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL647-20536

    IF Staining of HepG2 using CL647-20536

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 Beta Actin antibody (CL647-20536) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated Beta Actin Polyclonal antibody

Beta Actin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-20536

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine, monkey

应用

IF/ICC, FC (Intra)

别名

Actin, ACTB, bate actin, β actin, Beta-actin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ACTB Ready-To-Use IHC Kit. ACTB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 20536-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-20536 targets Beta Actin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, canine, monkey samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, canine, monkey
免疫原 Beta Actin fusion protein Ag14521 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 actin, beta
别名 Actin, ACTB, bate actin, β actin, Beta-actin
计算分子量 375 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC002409
基因名称 Beta Actin
Gene ID (NCBI) 60
RRIDAB_2934941
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60709
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Beta Actin antibody CL647-20536Download protocol
Standard Protocols
Click here to view our Standard Protocols