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- KD/KO Validated
Beta Actin Polyclonal antibody
Beta Actin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey, canine and More (9)
Applications
FC, IF, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
2198
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A549 cells, C6 cells, NIH/3T3 cells, mouse colon tissue |
| Positive IHC detected in | human colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MDCK cells |
| Positive FC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
产品信息
The immunogen of 20536-1-AP is Beta Actin Fusion Protein expressed in E. coli.
| Tested Applications | FC, IF, IHC, WB, ELISA |
| Cited Applications | CoIP, IF, IHC, IP, WB |
| Tested Reactivity | human, mouse, rat, monkey, canine |
| Cited Reactivity | bovine, Camelus bactrianus, canine, carp, chicken, duck, fish, goat, grouper, Hamster |
| Immunogen | Beta Actin fusion protein Ag14521 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | actin, beta |
| Synonyms | ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cytoskeleton Marker, F actin, PS1TP5BP1, β actin |
| Calculated molecular weight | 375 aa, 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC002409 |
| Gene symbol | ACTB |
| Uniprot | P60709 |
| Gene ID (NCBI) | 60 |
| RRID | AB_10700003 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Actin antibody 20536-1-AP | Download protocol |
| IHC protocol for Beta Actin antibody 20536-1-AP | Download protocol |
| IF protocol for Beta Actin antibody 20536-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. | ||
Cell Microglia jointly degrade fibrillar alpha-synuclein cargo by distribution through tunneling nanotubes. | ||
Immunity Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation. | ||
Cell Metab Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue. | ||
Cell Res Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells. |
