验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse spleen tissue, mouse heart tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
82469-6-RR targets Bcl2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Bcl2 fusion protein Ag24260 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | B-cell leukemia/lymphoma 2 |
| 别名 | Bcl-2, 240571H5, B cell leukemia/lymphoma 2, Bcl 2 |
| 计算分子量 | 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | NM-009741 |
| 基因名称 | Bcl2 |
| Gene ID (NCBI) | 12043 |
| RRID | AB_3670523 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P10417 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Bcl2 antibody 82469-6-RR | Download protocol |
| IHC protocol for Bcl2 antibody 82469-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
BMC Vet Res Cecropin AD ameliorates pneumonia and intestinal injury in mice with mycoplasma pneumoniae by mediating gut microbiota | ||
ACS Nano A Hybrid Manganese Nanoparticle Simultaneously Eliminates Cancer Stem Cells and Activates STING Pathway to Potentiate Cancer Immunotherapy |
