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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82469-6-RR (same clone as 82469-6-PBS)

    WB analysis using 82469-6-RR (same clone as 82469-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82469-6-RR (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 82469-6-RR (same clone as 82469-6-PBS)

    IHC staining of mouse spleen using 82469-6-RR (same clone as 82469-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82469-6-RR (Bcl2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 82469-6-RR (same clone as 82469-6-PBS)

    IHC staining of mouse spleen using 82469-6-RR (same clone as 82469-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82469-6-RR (Bcl2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 82469-6-RR (same clone as 82469-6-PBS)

    FC experiment of NIH/3T3 using 82469-6-RR (same clone as 82469-6-PBS)

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Bcl2 Recombinant antibody (82469-6-RR, Clone:240571H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82469-6-RR

    Affinity and Kinetic Characterization of 82469-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82469-6-RR against Mouse Bcl2 were performed. The affinity constant is 1.08 nM.

Mouse Bcl2 Recombinant antibody, PBS Only

Bcl2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 82469-6-PBS

产品说明书

CloneNo. 240571H5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, FC (Intra), ELISA

别名

Bcl-2, 240571H5, B cell leukemia/lymphoma 2, Bcl 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82469-6-PBS targets Bcl2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 mouse
免疫原 Bcl2 fusion protein Ag24260 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell leukemia/lymphoma 2
别名 Bcl-2, 240571H5, B cell leukemia/lymphoma 2, Bcl 2
计算分子量 26 kDa
观测分子量26 kDa
GenBank蛋白编号NM-009741
基因名称 Bcl2
Gene ID (NCBI) 12043
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP10417
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.