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验证数据展示

  • FC experiment of HeLa using CL647-66020

    FC experiment of HeLa using CL647-66020

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Bcl-XL (CL647-66020, Clone:4C12A6) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated BCL2L1 Monoclonal antibody

BCL2L1 Monoclonal Antibody for FC (Intra)
Cat No. CL647-66020

产品说明书

CloneNo. 4C12A6

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

Bcl-xL, 4C12A6, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66020-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66020 targets BCL2L1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 BCL2L1 fusion protein Ag18037 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 BCL2-like 1
别名 Bcl-xL, 4C12A6, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1
计算分子量 26 kDa
观测分子量 30 kDa
GenBank蛋白编号BC019307
基因名称 BCL2L1
Gene ID (NCBI) 598
RRIDAB_2935037
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07817
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 BCL2L1 antibody CL647-66020Download protocol
Standard Protocols
Click here to view our Standard Protocols