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验证数据展示

  • FC experiment of human PBMCs using CL594-98096

    FC experiment of human PBMCs using CL594-98096

    1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3), and 5 ul CoraLite®594 Anti-Human BTLA/CD272 Rabbit RecAb (CL594-98096, Clone: 241318H5) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL594-98096

    FC experiment of human PBMCs using CL594-98096

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human BTLA/CD272 Rabbit RecAb (CL594-98096, Clone: 241318H5) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

CoraLite®594 Anti-Human BTLA/CD272 Rabbit Recombinant Antibody

BTLA/CD272 Uni-rAb® Recombinant Antibody for FC
Cat No. CL594-98096

产品说明书

CloneNo. 241318H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

BTLA, 241318H5, B and T lymphocyte attenuator, B and T lymphocyte associated, B- and T-lymphocyte attenuator

缓冲液配方:  PBS, Azide, BSA
PBS, Azide, BSA
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 98096-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-98096 targets BTLA/CD272 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

Recombinant Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B and T lymphocyte associated
别名 BTLA, 241318H5, B and T lymphocyte attenuator, B and T lymphocyte associated, B- and T-lymphocyte attenuator
计算分子量 289 aa, 33 kDa
GenBank蛋白编号BC107091
基因名称 BTLA
Gene ID (NCBI) 151888
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z6A9
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

BTLA ( B and T lymphocyte attenuator), also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.

实验方案

Product Specific Protocols
FC protocol for CL594 BTLA/CD272 antibody CL594-98096Download protocol
Standard Protocols
Click here to view our Standard Protocols
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