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验证数据展示

  • FC experiment of human PBMCs using 98096-1-RR (same clone as 98096-1-PBS)

    FC experiment of human PBMCs using 98096-1-RR (same clone as 98096-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR, Clone: 241318H5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98096-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98096-1-RR (same clone as 98096-1-PBS)

    FC experiment of human PBMCs using 98096-1-RR (same clone as 98096-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR, Clone: 241318H5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98096-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98096-1-RR

    Affinity and Kinetic Characterization of 98096-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98096-1-RR against Human BTLA/CD272 were performed. The affinity constant is 1.84 nM.

Anti-Human BTLA/CD272 Rabbit Recombinant Antibody, PBS Only

BTLA/CD272 Uni-rAb® Recombinant Antibody for FC, Indirect ELISA
Cat No. 98096-1-PBS

产品说明书

CloneNo. 241318H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC, Indirect ELISA

别名

BTLA, 241318H5, B and T lymphocyte attenuator, B and T lymphocyte associated, B- and T-lymphocyte attenuator

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98096-1-PBS targets BTLA/CD272 in FC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 FC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Recombinant Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B and T lymphocyte associated
别名 BTLA, 241318H5, B and T lymphocyte attenuator, B and T lymphocyte associated, B- and T-lymphocyte attenuator
计算分子量 289 aa, 33 kDa
GenBank蛋白编号BC107091
基因名称 BTLA
Gene ID (NCBI) 151888
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ7Z6A9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BTLA ( B and T lymphocyte attenuator), also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.

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