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验证数据展示

  • FC experiment of mouse splenocytes using 98246-2-RR

    FC experiment of mouse splenocytes using 98246-2-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody (98246-2-RR, Clone: 242133A1) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2). Cells were not fixed.

  • FC experiment of mouse splenocytes using 98246-2-RR

    FC experiment of mouse splenocytes using 98246-2-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody (98246-2-RR, Clone: 242133A1)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98246-2-RR

    Affinity and Kinetic Characterization of 98246-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98246-2-RR against Mouse BTLA/CD272 were performed. The affinity constant is 0.441 nM.

Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody

BTLA/CD272 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98246-2-RR

产品说明书

CloneNo. 242133A1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

CD272, Btla, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, 242133A1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98246-2-RR targets BTLA/CD272 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B and T lymphocyte associated
别名 CD272, Btla, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, 242133A1
计算分子量34kDa
GenBank蛋白编号AAI08964.1
基因名称 Btla
Gene ID (NCBI) 208154
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ32MV9
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

BTLA, also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.

实验方案

Product Specific Protocols
FC protocol for BTLA/CD272 antibody 98246-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols