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验证数据展示

  • FC experiment of HeLa using CL488-30186

    FC experiment of HeLa using CL488-30186

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Brpf1 Polyclonal Antibody (CL488-30186)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated BRPF1 Polyclonal antibody

BRPF1 Polyclonal Antibody for FC (Intra)
Cat No. CL488-30186

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

FC (Intra)

别名

BR140, Bromodomain and PHD finger-containing protein 1, Peregrin, Protein Br140

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 30186-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-30186 targets BRPF1 in FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag32828

Product name: Recombinant human BRPF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 741-880 aa of BC053851

Sequence: QAEKMGIDFETGMHIPHSLAGDEATHHTEDAAEEERLVLLENQKHLPVEEQLKLLLERLDEVNASKQSVGRSRRAKMIKKEMTALRRKLAHQRETGRDGPERHGPSSRGSLTPHPAACDKDGQTDSAAEESSSQETSKGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bromodomain and PHD finger containing, 1
别名 BR140, Bromodomain and PHD finger-containing protein 1, Peregrin, Protein Br140
计算分子量 1220 aa, 138 kDa
观测分子量 140 kDa
GenBank蛋白编号BC053851
基因名称 BRPF1
Gene ID (NCBI) 7862
RRIDAB_3672844
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55201
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRPF1 (bromodomain and PHD finger-containing protein 1) is also named as BR140. BRPF1 is scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PMID:16387653, PMID:24065767, PMID:27939640). BRPF1 plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PMID:24065767). And BRPF1 positively regulates the transcription of RUNX1 and RUNX2 (PMID:18794358).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 BRPF1 antibody CL488-30186Download protocol
Standard Protocols
Click here to view our Standard Protocols
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