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BRPF1 Polyclonal antibody

BRPF1 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

FC, WB, ELISA

Conjugate

Unconjugated

Cat No : 30186-1-AP

Print datasheet

Synonyms

BR140, BRPF1, Peregrin, Protein Br140

验证数据展示

  • WB analysis using 30186-1-AP

    WB analysis using 30186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30186-1-AP (BRPF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 30186-1-AP

    FC experiment of HeLa using 30186-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human BRPF1 (30186-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, PC-12 cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30186-1-AP targets BRPF1 in WB, FC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications FC, WB, ELISA
Tested Reactivity Human, rat
Immunogen BRPF1 fusion protein Ag32828 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bromodomain and PHD finger containing, 1
Synonyms BR140, BRPF1, Peregrin, Protein Br140
Calculated Molecular Weight 1220 aa, 138 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC053851
Gene Symbol BRPF1
Gene ID (NCBI) 7862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55201
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRPF1 (bromodomain and PHD finger-containing protein 1) is also named as BR140. BRPF1 is scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PMID:16387653, PMID:24065767, PMID:27939640). BRPF1 plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PMID:24065767). And BRPF1 positively regulates the transcription of RUNX1 and RUNX2 (PMID:18794358).

实验方案

Product Specific Protocols
WB protocol for BRPF1 antibody 30186-1-APDownload protocol
FC protocol for BRPF1 antibody 30186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols