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验证数据展示

  • WB analysis using 32257-1-AP (same clone as 32257-1-PBS)

    WB analysis using 32257-1-AP (same clone as 32257-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32257-1-AP (BRN3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32257-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 32257-1-AP (same clone as 32257-1-PBS)

    IHC staining of mouse eye using 32257-1-AP (same clone as 32257-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 32257-1-AP (BRN3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32257-1-PBS in a different storage buffer formulation.

  • IF Staining of rat eye using 32257-1-AP (same clone as 32257-1-PBS)

    IF Staining of rat eye using 32257-1-AP (same clone as 32257-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using BRN3A antibody (32257-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32257-1-PBS in a different storage buffer formulation.

BRN3A Polyclonal antibody, PBS Only

BRN3A Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 32257-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

Brain-3A, Brain-specific homeobox/POU domain protein 3A, Brn-3A, Homeobox/POU domain protein RDC-1, Oct-T1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32257-1-PBS targets BRN3A in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35800

Product name: Recombinant human BRN3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 87-265 aa of NM_006237

Sequence: SVPCTSTSTVPLAHHHHHHHHHQALEPGDLLDHISSPSLALMAGAGGAGAAAGGGGAHDGPGGGGGPGGGGGPGGGPGGGGGGGPGGGGGGPGGGLLGGSAHPHPHMHSLGHLSHPAAAAAMNMPSGLPHPGLVAAAAHHGAAAAAAAAAAGQVAAASAAAAVVGAAGLASICDSDTDP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 POU class 4 homeobox 1
别名 Brain-3A, Brain-specific homeobox/POU domain protein 3A, Brn-3A, Homeobox/POU domain protein RDC-1, Oct-T1
计算分子量 43 kDa
观测分子量35 kDa
GenBank蛋白编号NM_006237
基因名称 POU4F1
Gene ID (NCBI) 5457
RRIDAB_3742554
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ01851
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BRN3A (Brain-specific Homeobox/POU domain protein 3A, also known as POU4F1) is a member of the class IV POU-domain transcription factor family. It plays a core regulatory role primarily in the development and survival of the nervous system and sensory neurons. By recognizing specific DNA sequences, it activates downstream target genes and is essential for the generation, differentiation, and maintenance of sensory ganglion neurons, including those of the trigeminal, vestibular, and auditory systems. Additionally, it is also involved in regulating cell proliferation and apoptosis in tumors such as breast cancer. It has 2 isoforms with the molecular mass of 33 kDa and 43 kDa.

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