验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat eye tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32257-1-AP targets BRN3A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag35800 Product name: Recombinant human BRN3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 87-265 aa of NM_006237 Sequence: SVPCTSTSTVPLAHHHHHHHHHQALEPGDLLDHISSPSLALMAGAGGAGAAAGGGGAHDGPGGGGGPGGGGGPGGGPGGGGGGGPGGGGGGPGGGLLGGSAHPHPHMHSLGHLSHPAAAAAMNMPSGLPHPGLVAAAAHHGAAAAAAAAAAGQVAAASAAAAVVGAAGLASICDSDTDP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | POU class 4 homeobox 1 |
| 别名 | Brain-3A, Brain-specific homeobox/POU domain protein 3A, Brn-3A, Homeobox/POU domain protein RDC-1, Oct-T1 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | NM_006237 |
| 基因名称 | POU4F1 |
| Gene ID (NCBI) | 5457 |
| RRID | AB_3742554 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q01851 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BRN3A (Brain-specific Homeobox/POU domain protein 3A, also known as POU4F1) is a member of the class IV POU-domain transcription factor family. It plays a core regulatory role primarily in the development and survival of the nervous system and sensory neurons. By recognizing specific DNA sequences, it activates downstream target genes and is essential for the generation, differentiation, and maintenance of sensory ganglion neurons, including those of the trigeminal, vestibular, and auditory systems. Additionally, it is also involved in regulating cell proliferation and apoptosis in tumors such as breast cancer. It has 2 isoforms with the molecular mass of 33 kDa and 43 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for BRN3A antibody 32257-1-AP | Download protocol |
| IHC protocol for BRN3A antibody 32257-1-AP | Download protocol |
| WB protocol for BRN3A antibody 32257-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
