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CoraLite® Plus 488-conjugated BOULE Polyclonal antibody

BOULE Polyclonal Antibody for IF-Fro,IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-Fro,IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-13720

Print datasheet

Synonyms

bol, boule like (Drosophila), BOLL, BOULE, Protein boule like

验证数据展示

  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:400, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite® Plus 647 TNP1 antibody (CL647-17178, Magenta).

  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:400, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite® Plus 647 TNP1 antibody (CL647-17178, Magenta).

  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (cold ethanol) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:100, CoraLite®555 TNP1 antibody (CL555-17178, orange), STAR antibody (67130-1-Ig, Clone: 1G6E6, Magenta).

经过测试的应用

Positive IF detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 13720-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13720 targets BOULE in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-Fro,IF-P
Tested Reactivity human, mouse, rat
Immunogen BOULE fusion protein Ag4662 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bol, boule-like (Drosophila)
Synonyms bol, boule like (Drosophila), BOLL, BOULE, Protein boule like
Calculated Molecular Weight 283 aa, 31 kDa
GenBank Accession NumberBC033674
Gene Symbol BOLL
Gene ID (NCBI) 66037
RRIDAB_2919076
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9W6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BOULE antibody CL488-13720Download protocol
Standard Protocols
Click here to view our Standard Protocols