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BLZF1 Polyclonal antibody

BLZF1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16354-1-AP

Print datasheet

Synonyms

BLZF1, GOLGIN 45, JEM 1, JEM 1s, JEM1

验证数据展示

  • WB analysis of K-562 using 16354-1-AP

    WB analysis of K-562 using 16354-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16354-1-AP

    IHC staining of human liver cancer using 16354-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 16354-1-AP

    IHC staining of human liver cancer using 16354-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 16354-1-AP is BLZF1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen BLZF1 fusion protein Ag9564 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name basic leucine zipper nuclear factor 1
Synonyms BLZF1, GOLGIN 45, JEM 1, JEM 1s, JEM1
Calculated Molecular Weight 400 aa, 45 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC020716
Gene Symbol BLZF1
Gene ID (NCBI) 8548
RRIDAB_2918045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2G9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BLZF1 (basic leucine zipper nuclear factor 1), also known as JEM1 or Golgin-45, maps to human chromosome 1q24, encodes for a 3 kb mRNA that is translated into a 45 kDa protein. BLZF1 is a member of b-ZIP family, and has been implicated in epigenetic regulation and Wnt/β-catenin signaling.

实验方案

Product Specific Protocols
WB protocol for BLZF1 antibody 16354-1-APDownload protocol
IHC protocol for BLZF1 antibody 16354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Biol Cell

Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function.

Authors - Michael E Bekier
View Article
humanWB

Methods Mol Biol

Immunofluorescence Microscopy of the Mammalian Golgi Apparatus

Authors - Maryam Arab
View Article