验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67260 targets BCR in FC (Intra) applications and shows reactivity with human, rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag28953 Product name: Recombinant human BCR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 286-494 aa of BC066122 Sequence: GMMEGEGKGPLLRSQSTSEQEKRLTWPRRSYSPRSFEDCGGGYTPDCSSNENLTSSEEDFSSGQSSRVSPSPTTYRMFRDKSRSPSQNSQQSFDSSSPPTPQCHKRHRHCPVVVSEATIVGVRKTGQIWPNDGEGAFHGDADGSFGTPPGYGCAADRAEEQRRHQDGLPYIDDSPSSSPHLSSKGRGSRDALVSGALESTKASELDLEK 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | breakpoint cluster region |
| 别名 | 2C2E3, BCR 1, BCR1, breakpoint cluster region, EC:2.7.11.1 |
| 计算分子量 | 143 kDa |
| 观测分子量 | 150-160 kDa |
| GenBank蛋白编号 | BC066122 |
| 基因名称 | BCR |
| Gene ID (NCBI) | 613 |
| RRID | AB_3084332 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11274 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 BCR antibody CL488-67260 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
