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CoraLite® Plus 488-conjugated BCL3 Polyclonal antibody
BCL3 Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 23959-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-23959 targets BCL3 in IF applications and shows reactivity with human, mouse samples.
| Tested Applications | IF |
| Tested Reactivity | human, mouse |
| Immunogen | BCL3 fusion protein Ag21040 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | B-cell CLL/lymphoma 3 |
| Synonyms | B cell CLL/lymphoma 3, B cell lymphoma 3 protein, BCL3, D19S37, Proto oncogene BCL3 |
| Calculated Molecular Weight | 446 aa, 47 kDa |
| Observed Molecular Weight | 48-60 kDa |
| GenBank Accession Number | BC064993 |
| Gene Symbol | BCL3 |
| Gene ID (NCBI) | 602 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20749 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCL3 (BCL3 Transcription Coactivator), as a Protein Coding gene, has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis. In the cytoplasm, BCL3 inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, BCL3 acts as transcriptional activator that promotes transcription of NF-kappa-B target genes(Uniprot).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 BCL3 antibody CL488-23959 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |