验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human lymphoma tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23959-1-AP targets BCL3 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP, IHC, CoIP, chIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | BCL3 fusion protein Ag21040 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | B-cell CLL/lymphoma 3 |
| 别名 | B cell CLL/lymphoma 3, B cell lymphoma 3 protein, BCL3, D19S37, Proto oncogene BCL3 |
| 计算分子量 | 446 aa, 47 kDa |
| 观测分子量 | 48-60 kDa |
| GenBank蛋白编号 | BC064993 |
| 基因名称 | BCL3 |
| Gene ID (NCBI) | 602 |
| RRID | AB_2879375 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20749 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCL3 (BCL3 Transcription Coactivator), as a Protein Coding gene, has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis. In the cytoplasm, BCL3 inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, BCL3 acts as transcriptional activator that promotes transcription of NF-kappa-B target genes(Uniprot).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BCL3 antibody 23959-1-AP | Download protocol |
| IHC protocol for BCL3 antibody 23959-1-AP | Download protocol |
| IF protocol for BCL3 antibody 23959-1-AP | Download protocol |
| IP protocol for BCL3 antibody 23959-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res SPaRTAN, a computational framework for linking cell-surface receptors to transcriptional regulators. | ||
Carcinogenesis Bcl-3 promotes multi-modal tumour cell migration via NF-κB1 mediated regulation of Cdc42.
| ||
J Biol Chem The proto-oncogene Bcl3 induces immune checkpoint PD-L1 expression, mediating proliferation of ovarian cancer cells.
| ||
Int J Oncol BCL‑3 promotes cyclooxygenase‑2/prostaglandin E2 signalling in colorectal cancer. |
