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验证数据展示

  • WB analysis using 85363-1-RR

    WB analysis using 85363-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85363-1-RR (BCL11B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 85363-1-RR

    IF Staining of Jurkat using 85363-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using BCL11B antibody (85363-1-RR, Clone: 242683B9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of Jurkat using 85363-1-RR

    FC experiment of Jurkat using 85363-1-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug BCL11B Recombinant antibody (85363-1-RR, Clone:242683B9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

BCL11B Recombinant monoclonal antibody

BCL11B Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85363-1-RR

产品说明书

CloneNo. 242683B9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

242683B9, B cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B-cell lymphoma/leukemia 11B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, MOLT-4 cells, mouse thymus tissue
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85363-1-RR targets BCL11B in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 11B (zinc finger protein)
别名 242683B9, B cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B-cell lymphoma/leukemia 11B
计算分子量 96 kDa
观测分子量116 kDa
GenBank蛋白编号NM_022898
基因名称 BCL11B
Gene ID (NCBI) 64919
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9C0K0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa.

实验方案

Product Specific Protocols
IF protocol for BCL11B antibody 85363-1-RRDownload protocol
WB protocol for BCL11B antibody 85363-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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