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验证数据展示

  • WB analysis using 85363-1-RR (same clone as 85363-1-PBS)

    WB analysis using 85363-1-RR (same clone as 85363-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85363-1-RR (BCL11B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85363-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 85363-1-RR (same clone as 85363-1-PBS)

    IF Staining of Jurkat using 85363-1-RR (same clone as 85363-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using BCL11B antibody (85363-1-RR, Clone: 242683B9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85363-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 85363-1-RR (same clone as 85363-1-PBS)

    FC experiment of Jurkat using 85363-1-RR (same clone as 85363-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug BCL11B Recombinant antibody (85363-1-RR, Clone:242683B9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85363-1-PBS in a different storage buffer formulation.

    {{ptg:ProductFeaturesInfo}}

BCL11B Recombinant monoclonal antibody, PBS Only

BCL11B Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85363-1-PBS

产品说明书

CloneNo. 242683B9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

242683B9, B cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B-cell lymphoma/leukemia 11B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85363-1-PBS targets BCL11B in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 11B (zinc finger protein)
别名 242683B9, B cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B-cell lymphoma/leukemia 11B
计算分子量 96 kDa
观测分子量116 kDa
GenBank蛋白编号NM_022898
基因名称 BCL11B
Gene ID (NCBI) 64919
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9C0K0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa.