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验证数据展示

  • WB analysis using 86367-1-RR

    WB analysis using 86367-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86367-1-RR (BCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 86367-1-RR

    IHC staining of human placenta using 86367-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86367-1-RR (BCAT2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 86367-1-RR

    IHC staining of human placenta using 86367-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86367-1-RR (BCAT2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 86367-1-RR

    Affinity and Kinetic Characterization of 86367-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86367-1-RR against Human BCAT2 were performed. The affinity constant is below 1 pM.

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BCAT2 Recombinant monoclonal antibody

BCAT2 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86367-1-RR

产品说明书

CloneNo. 251057E6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Branched-chain-amino-acid aminotransferase, mitochondrial, EC:2.6.1.42, ECA40, Placental protein 18, PP18

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, MCF-7 cells, Raji cells, HeLa cells, Jurkat cells, RAW 264.7 cells, PC-12 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86367-1-RR targets BCAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9573

Product name: Recombinant human BCAT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 41-392 aa of BC001900

Sequence: MTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 branched chain aminotransferase 2, mitochondrial
别名 Branched-chain-amino-acid aminotransferase, mitochondrial, EC:2.6.1.42, ECA40, Placental protein 18, PP18
计算分子量 44 kDa
观测分子量 38 kDa
GenBank蛋白编号BC001900
基因名称 BCAT2
Gene ID (NCBI) 587
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15382
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).

实验方案

Product Specific Protocols
WB protocol for BCAT2 antibody 86367-1-RRDownload protocol
IHC protocol for BCAT2 antibody 86367-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols