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验证数据展示

  • WB analysis using 86367-1-RR (same clone as 86367-1-PBS)

    WB analysis using 86367-1-RR (same clone as 86367-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86367-1-RR (BCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86367-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 86367-1-RR (same clone as 86367-1-PBS)

    IHC staining of human placenta using 86367-1-RR (same clone as 86367-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86367-1-RR (BCAT2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86367-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 86367-1-RR (same clone as 86367-1-PBS)

    IHC staining of human placenta using 86367-1-RR (same clone as 86367-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86367-1-RR (BCAT2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86367-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86367-1-RR

    Affinity and Kinetic Characterization of 86367-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86367-1-RR against Human BCAT2 were performed. The affinity constant is below 1 pM.

    {{ptg:ProductFeaturesInfo}}

BCAT2 Recombinant monoclonal antibody, PBS Only

BCAT2 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86367-1-PBS

产品说明书

CloneNo. 251057E6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Branched-chain-amino-acid aminotransferase, mitochondrial, EC:2.6.1.42, ECA40, Placental protein 18, PP18

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86367-1-PBS targets BCAT2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9573

Product name: Recombinant human BCAT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 41-392 aa of BC001900

Sequence: MTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 branched chain aminotransferase 2, mitochondrial
别名 Branched-chain-amino-acid aminotransferase, mitochondrial, EC:2.6.1.42, ECA40, Placental protein 18, PP18
计算分子量 44 kDa
观测分子量 38 kDa
GenBank蛋白编号BC001900
基因名称 BCAT2
Gene ID (NCBI) 587
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15382
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).