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验证数据展示

  • WB analysis using CL488-67084

    WB analysis using CL488-67084

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67084 (BCAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated BCAT1 Monoclonal antibody

BCAT1 Monoclonal Antibody for WB
Cat No. CL488-67084

产品说明书

CloneNo. 1G3A10

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB

别名

MECA39, ECA39, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, cytosolic, BCAT1/ECA39

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inDaudi cells
For other applications, we recommend the unconjugated version of this antibody, 67084-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67084 targets BCAT1 in WB applications and shows reactivity with human, pig samples.

经测试应用 WB Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag28328

Product name: Recombinant human BCAT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-320 aa of BC033864

Sequence: MDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWDTELSLFSINLPDFLQFIYF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 branched chain aminotransferase 1, cytosolic
别名 MECA39, ECA39, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, cytosolic, BCAT1/ECA39
计算分子量 320 aa, 36 kDa
观测分子量 42-45 kDa
GenBank蛋白编号BC033864
基因名称 BCAT1
Gene ID (NCBI) 586
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP54687
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 BCAT1 antibody CL488-67084Download protocol
Standard Protocols
Click here to view our Standard Protocols
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