验证数据展示
经过测试的应用
| Positive WB detected in | Ramos cells, Daudi cells, pig brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67084-1-Ig targets BCAT1 in WB, ELISA applications and shows reactivity with Human, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, pig |
| 免疫原 |
CatNo: Ag28328 Product name: Recombinant human BCAT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-320 aa of BC033864 Sequence: MDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWDTELSLFSINLPDFLQFIYF 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | branched chain aminotransferase 1, cytosolic |
| 别名 | BCAT1, DKFZp686E12175, ECA39, MECA39, PNAS 121, PP18, Protein ECA39 |
| 计算分子量 | 320 aa, 36 kDa |
| 观测分子量 | 42-45 kDa |
| GenBank蛋白编号 | BC033864 |
| 基因名称 | BCAT1 |
| Gene ID (NCBI) | 586 |
| RRID | AB_2882392 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P54687 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BCAT1 antibody 67084-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
