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验证数据展示

  • FC experiment of Human peripheral blood erythrocytes using 98521-3-RR (same clone as 98521-3-PBS)

    FC experiment of Human peripheral blood erythrocytes using 98521-3-RR (same clone as 98521-3-PBS)

    1x10^6 human peripheral blood erythrocytes were surface stained with 0.25 ug Anti-Human BCAM Rabbit RecAb (98521-3-RR, Clone: 250778C11) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98521-3-PBS in a different storage buffer formulation.

  • FC experiment of Human peripheral blood erythrocytes using 98521-3-RR (same clone as 98521-3-PBS)

    FC experiment of Human peripheral blood erythrocytes using 98521-3-RR (same clone as 98521-3-PBS)

    1x10^6 human peripheral blood erythrocytes were surface stained with 0.25 ug Anti-Human BCAM Rabbit RecAb (98521-3-RR, Clone: 250778C11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98521-3-PBS in a different storage buffer formulation.

  • IF Staining of HT-29 using 98521-3-RR (same clone as 98521-3-PBS)

    IF Staining of HT-29 using 98521-3-RR (same clone as 98521-3-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using BCAM antibody (98521-3-RR, Clone: 250778C11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 98521-3-PBS in a different storage buffer formulation.

Anti-Human BCAM Rabbit Recombinant Antibody, PBS Only

BCAM Uni-rAb® Recombinant Antibody for IF/ICC, FC
Cat No. 98521-3-PBS

产品说明书

CloneNo. 250778C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC

别名

Auberger B antigen, Basal cell adhesion molecule, B-CAM cell surface glycoprotein, CD239, F8/G253 antigen

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98521-3-PBS targets BCAM in IF/ICC, FC applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 human
免疫原

CatNo: Eg2567

Product name: Recombinant Human BCAM protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 32-547 aa of BC050450

Sequence: EVRLSVPPLVEVMRGKSVILDCTPTGTHDHYMLEWFLTDRSGARPRLASAEMQGSELQVTMHDTRGRSPPYQLDSQGRLVLAEAQVGDERDYVCVVRAGAAGTAEATARLNVFAKPEATEVSPNKGTLSVMEDSAQEIATCNSRNGNPAPKITWYRNGQRLEVPVEMNPEGYMTSRTVREASGLLSLTSTLYLRLRKDDRDASFHCAAHYSLPEGRHGRLDSPTFHLTLHYPTEHVQFWVGSPSTPAGWVREGDTVQLLCRGDGSPSPEYTLFRLQDEQEEVLNVNLEGNLTLEGVTRGQSGTYGCRVEDYDAADDVQLSKTLELRVAYLDPLELSEGKVLSLPLNSSAVVNCSVHGLPTPALRWTKDSTPLGDGPMLSLSSITFDSNGTYVCEASLPTVPVLSRTQNFTLLVQGSPELKTAEIEPKADGSWREGDEVTLICSARGHPDPKLSWSQLGGSPAEPIPGRQGWVSSSLTLKVTSALSRDGISCEASNPHGNKRHVFHFGTVSPQTSQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 basal cell adhesion molecule (Lutheran blood group)
别名 Auberger B antigen, Basal cell adhesion molecule, B-CAM cell surface glycoprotein, CD239, F8/G253 antigen
计算分子量 67 kDa
GenBank蛋白编号BC050450
基因名称 BCAM
Gene ID (NCBI) 4059
RRIDAB_3746149
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP50895
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Basal cell adhesion molecule (Lu/BCAM) is a membrane-bound glycoprotein of the immunoglobulin superfamily (IgSF), functioning as a receptor for the extracellular matrix protein, laminin. BCAM (Lutheran/basal cell-adhesion molecule, a glycoprotein) belongs to the immunoglobulin superfamily which contains both Lu blood group and BCAM tumor-associated antigens. Proteomics analysis suggests that BCAM might be a potential biomarker for pancreatic cancer (PMID: 19199705). BCAM, involved in cell adhesion and migration, can also promote tumor metastasis and has been elucidated to play a functional role in the metastasis of thyroid cancer and gastric cancer (PMID: 10728810, 35941663).

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