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验证数据展示

  • WB analysis using 83596-4-RR (same clone as 83596-4-PBS)

    WB analysis using 83596-4-RR (same clone as 83596-4-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83596-4-RR (BARHL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

    IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

    IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83596-4-RR

    Affinity and Kinetic Characterization of 83596-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83596-4-RR against Human BARHL2 were performed. The affinity constant is 0.478 nM.

BARHL2 Recombinant monoclonal antibody, PBS Only

BARHL2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 83596-4-PBS

产品说明书

CloneNo. 240558G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

240558G8, BarH like 2 homeobox protein, BarH-like 2 homeobox protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83596-4-PBS targets BARHL2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21156

Product name: Recombinant human BARHL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-141 aa of BC126439

Sequence: MTMEGASGSSFGIDTILSSASSGSPGMMNGDFRPLGEARTADFRSQATPSPCSEIDTVGTAPSSPISVTMEPPEPHLVADATQHHHHLHHSQQPPPPAAAPTQSLQPLPQQQQPLPPQQPPPPPPQQLGSAASAPRTSTSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BarH-like homeobox 2
别名 240558G8, BarH like 2 homeobox protein, BarH-like 2 homeobox protein
计算分子量 387 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC126439
基因名称 BARHL2
Gene ID (NCBI) 343472
RRIDAB_3671212
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NY43
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BARHL2 also named as BarH like homeobox 2 is 279 amino acid protein, which contains 1 homeobox DNA-binding domain. BARHL2 localizes in nucleus and belongs to BAR homeobox family. BARHL2 as a transcription factor binds optimally to the DNA consensus sequence. Barx2 may differentially control the expression of L1 and other target genes during embryonic development. Barx2 is expressed in several smooth muscle-containing tissues, as well as skeletal muscle, brain, tongue and esophagus.

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