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验证数据展示

  • WB analysis using 83596-4-RR

    WB analysis using 83596-4-RR

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83596-4-RR (BARHL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83596-4-RR

    IF Staining of HepG2 using 83596-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 83596-4-RR

    IF Staining of HepG2 using 83596-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83596-4-RR

    Affinity and Kinetic Characterization of 83596-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83596-4-RR against Human BARHL2 were performed. The affinity constant is 0.478 nM.

BARHL2 Recombinant antibody

BARHL2 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83596-4-RR

产品说明书

CloneNo. 240558G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

BarH-like 2 homeobox protein, BarH like 2 homeobox protein, 240558G8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83596-4-RR targets BARHL2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BARHL2 fusion protein Ag21156 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BarH-like homeobox 2
别名 BarH-like 2 homeobox protein, BarH like 2 homeobox protein, 240558G8
计算分子量 387 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC126439
基因名称 BARHL2
Gene ID (NCBI) 343472
RRIDAB_3671212
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NY43
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BARHL2 also named as BarH like homeobox 2 is 279 amino acid protein, which contains 1 homeobox DNA-binding domain. BARHL2 localizes in nucleus and belongs to BAR homeobox family. BARHL2 as a transcription factor binds optimally to the DNA consensus sequence. Barx2 may differentially control the expression of L1 and other target genes during embryonic development. Barx2 is expressed in several smooth muscle-containing tissues, as well as skeletal muscle, brain, tongue and esophagus.

实验方案

Product Specific Protocols
WB protocol for BARHL2 antibody 83596-4-RRDownload protocol
IF protocol for BARHL2 antibody 83596-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols