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验证数据展示

  • IF Staining of mouse brain using CL594-82296-6

    IF Staining of mouse brain using CL594-82296-6

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite®594 Aquaporin 4 antibody (CL594-82296-6, Clone: 240856C7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using CL594-82296-6

    IF Staining of mouse brain using CL594-82296-6

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite®594 Aquaporin 4 antibody (CL594-82296-6, Clone: 240856C7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CoraLite®594-conjugated Aquaporin 4 Recombinant monoclonal antibody

Aquaporin 4 Uni-rAb® Recombinant Antibody for IF-P
Cat No. CL594-82296-6

产品说明书

CloneNo. 240856C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

AQP4, 240856C7, AQP 4, AQP-4, Aquaporin4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 82296-6-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-82296-6 targets Aquaporin 4 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9561

Product name:

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: of

Sequence:

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Aquaporin 4
别名 AQP4, 240856C7, AQP 4, AQP-4, Aquaporin4
计算分子量 323 aa, 35 kDa
观测分子量 32-34 kDa
GenBank蛋白编号BC022286
基因名称 Aquaporin 4
Gene ID (NCBI) 361
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP55087
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

实验方案

Product Specific Protocols
IF protocol for CL594 Aquaporin 4 antibody CL594-82296-6Download protocol
Standard Protocols
Click here to view our Standard Protocols