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验证数据展示

  • IHC staining of rat brain using 82296-6-RR (same clone as 82296-6-PBS)

    IHC staining of rat brain using 82296-6-RR (same clone as 82296-6-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82296-6-PBS in a different storage buffer formulation.

  • WB analysis using 82296-6-RR (same clone as 82296-6-PBS)

    WB analysis using 82296-6-RR (same clone as 82296-6-PBS)

    37°C incubated mouse brain tissue were subjected to SDS PAGE followed by western blot with 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82296-6-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 82296-6-RR (same clone as 82296-6-PBS)

    IHC staining of rat brain using 82296-6-RR (same clone as 82296-6-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82296-6-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 82296-6-RR (same clone as 82296-6-PBS)

    IF Staining of mouse brain using 82296-6-RR (same clone as 82296-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Aquaporin 4 antibody (82296-6-RR, Clone: 240856C7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82296-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82296-6-RR

    Affinity and Kinetic Characterization of 82296-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82296-6-RR against Human Aquaporin 4 were performed. The affinity constant is 5.06 nM.

Aquaporin 4 Recombinant monoclonal antibody, PBS Only

Aquaporin 4 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 82296-6-PBS

产品说明书

CloneNo. 240856C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

AQP4, 240856C7, AQP 4, AQP-4, Aquaporin4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82296-6-PBS targets Aquaporin 4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9561

Product name: Recombinant human AQP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 208-323 aa of BC022286

Sequence: TGASMNPARSFGPAVIMGNWENHWIYWVGPIIGAVLAGGLYEYVFCPDVEFKRRFKEAFSKAAQQTKGSYMEVEDNRSQVETDDLILKPGVVHVIDVDRGEEKKGKDQSGEVLSSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Aquaporin 4
别名 AQP4, 240856C7, AQP 4, AQP-4, Aquaporin4
计算分子量 323 aa, 35 kDa
观测分子量 32-34 kDa
GenBank蛋白编号BC022286
基因名称 Aquaporin 4
Gene ID (NCBI) 361
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP55087
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

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