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验证数据展示

  • IHC staining of rat brain using 82296-6-RR

    IHC staining of rat brain using 82296-6-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 82296-6-RR

    WB analysis using 82296-6-RR

    37°C incubated mouse brain tissue were subjected to SDS PAGE followed by western blot with 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 82296-6-RR

    IHC staining of rat brain using 82296-6-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 82296-6-RR

    IF Staining of mouse brain using 82296-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Aquaporin 4 antibody (82296-6-RR, Clone: 240856C7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82296-6-RR

    Affinity and Kinetic Characterization of 82296-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82296-6-RR against Human Aquaporin 4 were performed. The affinity constant is 5.06 nM.

Aquaporin 4 Recombinant monoclonal antibody

Aquaporin 4 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 82296-6-RR

产品说明书

CloneNo. 240856C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

AQP4, 240856C7, AQP 4, AQP-4, Aquaporin4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格价格库存

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经过测试的应用

Positive WB detected in37°C incubated mouse brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82296-6-RR targets Aquaporin 4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9561

Product name: Recombinant human AQP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 208-323 aa of BC022286

Sequence: TGASMNPARSFGPAVIMGNWENHWIYWVGPIIGAVLAGGLYEYVFCPDVEFKRRFKEAFSKAAQQTKGSYMEVEDNRSQVETDDLILKPGVVHVIDVDRGEEKKGKDQSGEVLSSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Aquaporin 4
别名 AQP4, 240856C7, AQP 4, AQP-4, Aquaporin4
计算分子量 323 aa, 35 kDa
观测分子量 32-34 kDa
GenBank蛋白编号BC022286
基因名称 Aquaporin 4
Gene ID (NCBI) 361
RRIDAB_3670521
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP55087
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.

实验方案

Product Specific Protocols
IF protocol for Aquaporin 4 antibody 82296-6-RRDownload protocol
IHC protocol for Aquaporin 4 antibody 82296-6-RRDownload protocol
WB protocol for Aquaporin 4 antibody 82296-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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