验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-68618 targets Angiopoietin 1 in IF/ICC applications and shows reactivity with human, mouse samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse |
免疫原 | Angiopoietin 1 fusion protein Ag18829 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | angiopoietin 1 |
别名 | |
计算分子量 | 498 aa, 58 kDa |
观测分子量 | 70 kDa, 55 kDa |
GenBank蛋白编号 | BC152411 |
基因名称 | Angiopoietin 1 |
Gene ID (NCBI) | 284 |
RRID | AB_3673026 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q15389 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Angiopoietin 1 antibody CL488-68618 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |