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验证数据展示

  • IF Staining of HeLa using CL488-68618

    IF Staining of HeLa using CL488-68618

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Angiopoietin 1 antibody (CL488-68618, Clone: 3C4A3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Angiopoietin 1 Monoclonal antibody

Angiopoietin 1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68618

产品说明书

CloneNo. 3C4A3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC

别名

ANGPT1, 3C4A3, AGP1, AGPT, ANG 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68618-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68618 targets Angiopoietin 1 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 Angiopoietin 1 fusion protein Ag18829 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 angiopoietin 1
别名 ANGPT1, 3C4A3, AGP1, AGPT, ANG 1
计算分子量 498 aa, 58 kDa
观测分子量70 kDa, 55 kDa
GenBank蛋白编号BC152411
基因名称 Angiopoietin 1
Gene ID (NCBI) 284
RRIDAB_3673026
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15389
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Angiopoietin 1 antibody CL488-68618Download protocol
Standard Protocols
Click here to view our Standard Protocols